Tissue-specific co-expression and in vitro heteropolymer formation of the two small Branchiostoma intermediate filament proteins A3 and B2
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چکیده
منابع مشابه
Tissue-specific co-expression and in vitro heteropolymer formation of the two small branchiostoma intermediate filament proteins A3 and B2.
The two small intermediate filament (IF) proteins A3 and B2 of the cephalochordate Amphioxus were investigated. Blot overlays indicated a heterotypic interaction pattern of the recombinant proteins. While the individual proteins formed only aggregates, the stoichiometric mixture formed obligatory heteropolymeric filaments. Mutant proteins with a single cysteine residue in equivalent positions g...
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The cloning of three intermediate filament proteins expressed at the gastrula stage (kl, Y1, X1) extends the size of the IF multigene family of Branchiostoma to at least 13 members. This is one of the largest protein families established for the lancelet. Sequence comparisons indicate five keratin orthologs, three of type I (E1, k1, Y1) and two of type II (E2, D1). This assignment is confirmed ...
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چکیده ندارد.
15 صفحه اولDevelopmentally controlled expression patterns of intermediate filament proteins in the cephalochordate Branchiostoma
Expression of cytoplasmic intermediate filament (IF) proteins starts in the gastrula with three keratins (k1, Y1, D1) and protein X1. The number of IF proteins expressed increases at the neurula and early larval stages to seven and 11, respectively, and reaches 13 in the adult. Using antibodies specific for a single IF protein the expression patterns of nine of the 13 IF proteins were analyzed ...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2002
ISSN: 0022-2836
DOI: 10.1006/jmbi.2001.5298